BFBThemeNights
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November 25th 2021
Mass Spectrometry
MARC SYLVESTER (CORE FACILITY MANAGER MASS SPECTROMETRY)
Felix Meissner (PI, INSTITUTE OF INNATE IMMUNITY)
Susanne Schoch McGovern (PI, DEPARTMENT OF NEUROPATHOLOGY)

OCTOBER 21st 2021
NGS and BIOINFORMATICS
Andreas Buness, Manager Core Facility for Bioinformatics Data Analysis
Maike Adamson, Clinical Chemistry, Clinical Pharmacology, UKB: "Trained Immunity in Nucleic Acid Sensing“
André Heimbach, Manager Core Facility for Next Generation Sequencing
Susanne Schmidt, Institute of Innate Immunity, Medical Faculty, University of Bonn:
"Altered histone modifications in DIPG brain tumors“

SEPTEMBER 23rd 2021
Gene Editing and Cell Programing
Dr. Simon Schneider, Manager Core Facility Gene Editing
Dr. Michael Peitz, Manager Core Facility Cell Programming
Prof. Dr. Jonathan Schmid-Burgk, Institute for Clinical Chemistry
and Clinical Pharmacology, Functional Immunogenomics
Prof. Dr. Volker Busskamp, Department of Ophthalmology

June 17th 2021
SARS CoV2 From virus to vaccination
Dr. Thomas Frischmuth,CEO baseclick GmbH: The Corona
Pandemic and vaccination – Status June 2021
Dr. Simon Schröder, Virologe AG Drosten, Charité: From
genotype to phenotype - reverse genetics as a tool to study
coronavirus diversity
Prof. Dr. Clara Lehmann, MVZ, Uniklinik Köln: CoronaImpfstoff: neue Technik- neueRisiken


December 3rd 2020
Single-cell transcriptomics
Dr. Tal Pecht, Systems Medicine, DZNE & Genomics & Immunoregulation, LIMES Institut
The power of one - Introduction to single-cell RNA-seq
Dr. Kristian Händler, PRECISE Platform for Genomics and Epigenomics at DZNE 
Kevin Baßler, Systems Medicine, DZNE & Genomics & Immunoregulation, LIMES Institut
A glimpse into the bioinformatics toolbox for scRNA-seq analyses
Comma Soft Team: FASTGenomics – a platform for single cell transcriptomics
Dr. Anna Aschenbrenner, Systems Medicine, DZNE & Genomics & Immunoregulation,
LIMES Institut: Application of scRNA-seq to study diseases
Dr. Antonella Succurro, WGGC Officer at the LIMES Institute : Next Generation Sequencing - where to start? Your NGS project with the West German Genome Center

November 19th 2020
Organoide
Marieta Toma (Institute of Pathology): Organoid technology for disease modeling
Kritika Sharma (Eye clinic): Stem cell technology and retinal organoids
Florian Schmidt/Florian Gohr (Institute of Innate Immunity): Intestinal Organoids for Infection Biology and Immunology


April 29, 2020 online
FACS
Andreas Dolf: FACS technique and the core facility
Dr. Zeinab Abdullah: Ongoing COVID projects

October 24, 2019
Imaging
Ireen König: “Examples of advanced light microscopy methods – microdissection, live cell imaging and FRAP”
Hannes Beckert: “Fluorescence imaging with confocal and spinning disk mircoscopes”
Ulrich Kubitscheck: “Combining light sheet imaging and expansion microscopy -> fast optical slicing at super resolution”
Stephan Irsen “3D Electron microscopy of large volumes - how to image whole brains in the electron microscope”

Juni 27, 2019
CRISPR
Gina Esther Merges, Institute of Pathology, Department of Developmental Pathology
Introduction
Maike Effern, Institute of Clincal Chemistry and Clinical Pharmacology
CRISPitope: a versatile approach to model antigen recognition of endogenous target proteins by T cells
Kai Funke, Institute of Pathology, Department of Developmental Pathology
Insights into genome-wide CRISPR/Cas9 library screening
PD Dr. Heike Weighardt, LIMES, Unit: Immunology and Environment

November 19, 2018
BFBThemeNight Mass Spectometry
Marc Sylvester, Institute of Biochemistry and Molecular Biology, Core Facility Mass spectometry
Peter Schein, Institute of Biochemistry and Molecular Biology
Andreas Buness, Institute of Medical Biometry, Informatics and Epidemiology, Core Unit for Bioinformatics Data Analysis

September 7, 2017
BFBThemeNight Behavioral Experiments
Dr. Andras Bilkei-Gorzo, Institute of Molecular Psychiatry: Animal Models in neuroscience research.
Dr. Ildiko Racz , Clinic for Neurodegenerative Disease and Gerontopsychiatry / Neurology: Can a mouse be alcohol dependent?
Dr. Sebastian Hückesfeld, LIMES: From behavior to neural circuit function in Drosophila larvae
Lothar Baltruschat, Ph.D, DZNE
Malou Zeymer, Sarah Pannhausen, Institute of Zoology: Living in the dark: Orientation and Communication in weakly electric fish

July 6, 2017
BFBThemeNight Intravital Micrsocopy
Kevin Keppler (Light Microscope Facility of the DZNE) An introduction to intra vital microscopy and the application of two-photon microscopy in neuroscience
Liu Changsheng (Institute of Anatomy) In vivo 2-photon time-lapse imaging in zebrafish
Alexander Harder (Institut für Physikalische und Theoretische Chemie)
In vivo two-photon light sheet imaging in zebrafish larvae
Johannes Seelig (Caesar) Neuromodulators on circuits underlying sensorimotor integration

Mai 25, 2017
BFBThemeNightFlow Cytometry
Andreas Dolf (FACS Core Facility Manager) Introduction
Laia Civit (LIMES, Center of Aptamer Research & Development) Flow cytometry in aptamer research
Natalio Garbi (IEI) Flow cytometric multiplexed assay to evaluate functional maturation of cytotoxic CD8 T cells in vivo
Florian Hoß (Institute of Innate Immunity) Visualisation of protein aggregation and re-localization in cells
Julia Reinhardt (Inst. clin. chem. and clin. pharmac.) A FACS-Sorting approach for CRISPR/Cas9-based functional Enhancer-Mapping
Ann-Kathrin Baumgart (IEI) Analysis of CD8+ T cell differentiation by single cell sorting and multiplex PCR

März 23, 2017
BFBThemeNight Berufsfelder in der Life Sciences Industrie
Carolin Kutzki, Marketing Manager, Cell Signaling Technology

Januar 12, 2017
BFBThemeNight Protein Expression
Lukas Bethlehem (AG Galinski - IFMB)
Tobias Zbik (Molecular Sensory Systems - caesar)
Matthias Zehner (Cellular Immunology - LIMES)
Robert Düster (Structural Immunology - caesar)

November 24, 2016
BFBThemeNight Funding
Dr. Beate Scholz, Consulting – Training - Coaching

October 11 2016
BFBThemeNight Good Scientific Practice
Dr. P. Schröder, brain4hire, Düsseldorf
Prof. Dr. U. B. Kaupp, Director research center caesar, Bonn
Dr. B. Pulverer, Chief Editor The EMBO Journal, Head EMBO Scientific Publications, Heidelberg

July 21 2016
BFBThemeNight Single Cell Analysis
Dr. Andreas Schlitzer, LIMES
Novel developments in single cell biology - From complex tissues to single cells
Patrick Günther, LIMES
Computational approaches to analyze Single-Cell RNA-Seq Data
Dr. Kristian Händler, LIMES
Single-Cell RNA-Seq Library Production

June 23 2016
BFBThemeNight Optogenetics and Patch Clamp
Prof. Dr. Benjamin Kaupp, caesar,
Optogenetics and Patch Clamping – an Introduction
Dr. Thomas Berger, Caesar
Patch-Clamp Recordings
Dr. Jan Jikeli, Caesar
Optogenetics- controlling cell function with light
Dr. Oliver Braganza, The Laboratory for Experimental Epileptology
Illuminating neuronal networks

October 22 2015
BFBThemeNight Image Analysis
Prof. Thorsten Lang (LIMES Institute – Department of Membrane
Biochemistry)
Introduction to Imaging Techniques
Manuel Schölling (DZNE Image and Data Analysis Core Facility)
Bioimage analysis - from small pixels to big data
Dr. Gabor Horvath (Institute of Innate Immunity Imaging Core Facility)
Quantitative Image Analysis in Biophysical Measurements
Dr. Andreas Till (Institute of Reconstructive Neurobiology – Stem Cell Pathologies group)
Single-cell based Image Analysis: Examples from Inflammation Research, Neurobiology and Oncology

September 17 2015
BFBThemeNight Model Organisms
Mice/Rats HET Dr. W. Eichelkraut
Zebrafish; Benjamin Odermatt, Institute of Anatomy
Drosophila; Marie Paradis, Yanina-Yasmin Pesch, Limes
Worms; DZNE AG Bano

July 23 2015
BFBThemeNight EM
Stephan Irsen: Electron Microscopy
Elmar Behrmann: Single Particle Reconstruction
Ashraf Al-Amoudi: Electron Tomography

March 19 2015
BFBThemeNight CRISPR/CAS
Dr. Marc Beyer, LIMES, AG Schultze, Genome engineering to optimize generation of animal models
Mirco Brondolin, LIMES, AG Hoch, Generating a mutant zebrafish line using CRISPR/Cas9 system: tricks and tips from A to Z
Thomas Ebert, IMM, AG Hornung, Gearing up to high throughput KO generation
Jonathan Schmid-Burgk, IMM, AG Hornung, OutKnocker.org: Online genotyping of knock-out cell lines



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